Yang Rui stayed in the sanatorium and set up the equipment with Liu Shan.

There are many ways to isolate strains, in 1879, the French isolated high-temperature strains from the Seine, after more than 100 years of development, the processing methods of samples have long been diverse, the difference between the times, more in the way of simplification, improve the yield rate.

Of course, the cost also increases.

Yang Rui first prepared a centrifuge - a machine commonly used in the biological chamber that can no longer be used, for centrifuging water and mud samples.

Under the action of a centrifuge of 10,000 times per minute, the water sample directly separates the impurities and supernatant that enrich the bacteria.

The isolated cells are relatively large, and the supernatant will also be eluted out of the cells through the guò filter membrane.

They are then cultured in a culture medium and are ready for screening.

The principle of mud sample is also similar, except that it needs to be suspended with spring water first, and then centrifuged at low speed to remove large particles, and then treated and enriched bacteria in the way of water samples.

The whole process, in theory, is very simple, but the equipment is not simple.

The centrifuge that can turn 10,000 times per minute is not cheap, and the capacity is smaller.

The culture stage is also very expensive, and the agar used in the culture medium, glucose, peptone, etc., are much more expensive than the same weight of food.

In addition, the cultivation conditions of 70 degrees or even above 80 degrees also put great pressure on the cultivation cost.

Kang Hong helped Yang Rui transport equipment and materials, and when he opened it, the little remaining flame of competition was also extinguished.

He didn't want to compete in the first place, and isolating strains is not a new technology, the only thing that makes sense is what strains can be isolated.

If you isolate a powerful strain, you can naturally invent a paper on cow breaking, but if you isolate only an ordinary strain, it will be difficult to get into an entry journal for SCI.

The results are as ethereal as buying a lottery ticket, but the cost of Yang Rui's investment is so high. When Kang Hong saw it, he felt that it lacked competitive value.

"Is it worth spending so much money to isolate thermophilic bacteria?"

"The instruments are not bad, and they will still work when we increase the number of people. Yang Rui replied while plugging in the wire.

Kang Hong picked up a large box of filter membranes, put them in the corner, and said, "What about consumables." These are all ultrafiltration membranes, all of them are imported, so do you need to use so much?"

Yang Rui said "En" and said: "I originally planned to process a batch first, and then bring back a batch, these costs cannot be saved." ”

"It's too expensive. We can't afford it. Kang Hong sighed twice.

"If you're lucky, there's still a little left. Yang Rui said casually, and Liu Shan assembled the incubator.

There are many kinds of filter membranes, and ordinary people are more familiar with them, which should be the filter element in the home water purifier, which is also a kind of filter membrane, in the initial stage. These civilian products are all from the real yàn room, and in the 80s, naturally they were very valuable.

In fact, the real yàn room has higher requirements for materials, and it is not cheap at any time.

In order to isolate the thermophilic bacteria he wanted as soon as possible, Yang Rui was very willing to spend money, and Kang Hong had no reason to make such a large investment, so he naturally couldn't understand it. It can only be attributed to the fact that the people at Jingcheng University are stupid and have a lot of money.

Yang Rui had no choice but to do so.

Isolating a strain is a relatively common research task, but isolating the desired strain is not.

Historically, most antibiotics have come from the isolation of soil strains, and for two or three decades, biologists have destroyed almost all of the world's soils, the most successful of which is Waxman, the terminator of tuberculosis, who invented a systematic isolation method. Then he got streptomycin, and then he got the Nobel Prize.

Waxman organized up to 50 research dogs for this purpose, and it took several years to screen more than 10,000 strains. I got the one I wanted......

Yang Rui can't afford to raise 50 real dogs, and he doesn't have a few years to waste, but fortunately, he knows for sure which hot spring it is, and as long as the number of separations is enough, he can always find the breed he wants.

A simple real room took more than half a day to install.

Yang Rui casually grabbed a handful of soil from the ground, mixed it with some liquid, loaded it into a centrifuge, and tested it.

The 10,000-rpm centrifuge is very noisy, but it is loud Kang Hong's heart is full of joy.

"We also have one in our yard, which is locked up with a wooden cabinet. Kang Hong stared at the machine qì in the transparent glass, and suddenly said.

Yang Rui understood: "The school's public real room is like this. ”

"Peking University too?" Now the information is backward, and the distance from Yunnan to Beijing is farther than from Beijing to Los Angeles, and it is almost as fast as Fuzhou to Taipei.

Yang Rui recalled for a moment and said, "Almost, as long as the administrator of the public real room has good instruments, he doesn't care if it's convenient for you to use it or not." ”

"That is to say, we apply for a good machine, at least two months in advance, and if we don't get it, we have to treat them, and when it's over, people still say how many false invoices we have, what invoices we falsely issue, all for these grandchildren to eat. "Any researcher who talks about the use of instruments or anything is full of fire.

When Yang Rui was a graduate student, he was also afraid of using the instruments of the public real room, of course, what he was most afraid of was borrowing the instruments of other real rooms, looking at other people's eyes was uncomfortable, and rubbing the equipment under the noses of others was even more uncomfortable.

This kind of pain, but anyone who has been in the real yàn room for half a year, has a deep experience, Yang Rui tried every means to make money, and half of the reason for building his own real yàn room was to avoid the recurrence of the dilemma.

If it's just to meet personal expenses, where can he use such trouble, buy a monkey ticket for 2,000 yuan, and when it's almost time to throw away the house, and become a billionaire in the 21st century casually.

However, it is not as interesting as being a billionaire to decide on his own research direction and do it on his own.

If there is no huge pressure and personnel struggles, as the person in charge of a real office, it is quite rewarding to do the scientific research you want to do and really do it.

Kang Hong had no experience with Yang Rui, and he didn't know how to get rid of the current restrictions, so he just asked enviously: "Then where did you get the instrument you took out?

"It's funded by a Hong Kong company. ”

"Hong Kong people are really rich. Yasuhiro asked, "Where did you meet them?"

"I wrote the paper and they were interested...... Well, the centrifuge stopped, and it looks good. Yang Rui took this opportunity to leave and got busy.

Liu Shan smiled at Kang Hong and began to give Yang Rui a hand.

Yang Rui slowly became serious.

It's not worth it to isolate an ordinary strain, but if you isolate an extreme thermophilic bacterium, and then isolate the thermostable polymerase inside, it's more or less a good paper.

Impact Factor 4. 0 "JMC" is probably in front of it, and if the goal is lowered a little bit, it must be no problem.

Theoretically, it makes sense to spend 600,000 yuan for such a paper.

Although it is reasonable to say that it is less cost-effective, considering that it is only a necessary step for the thesis, it is still not a loss.

Kang Hong saw that they were busy, so he had to say goodbye in a huff.

Yang Rui and Liu Shan did not complete the preparations until the afternoon was cool down.

"Haven't come back yet, probably camping outside. What are we going to eat at night?" Yang Rui looked at his watch, camping was planned, the hot vents were in an undeveloped state, and they had only done a simple geological survey, so they might not be able to go back and forth in a day.

Liu Shan tilted her head and thought for a while, then raised her hand and said, "Eat instant noodles." ”

"Instant noodles? Okay, just right with hot spring eggs. Yang Rui cleaned up and went to the cafeteria to buy eggs and instant noodles and came back.

At this time, instant noodles are still a fresh product that the old cadres want to try.

"Where are you going to eat?" Yang Rui asked, and then said, "I'll find a waiter and ask him to scald eggs for us." ”

"Let's go. Liu Shan carried a small bag, blinked her eyes, and said, "Let's make our own hot spring eggs, you can soak in the hot springs, eat instant noodles at the same time, and eat eggs at the same time." ”

Yang Rui said in surprise: "Men and women take separate baths." ”

"That little yard is empty. Liu Shan pointed to the courtyard at the foot of the mountain not far away.

At the entrance of the courtyard hangs a sign that reads "Premium Hot Spring Room", which is a small courtyard built around a hot spring pool. The hot springs that have been introduced are steaming and can be enjoyed by guests day and night, without the need for multiple changing rooms.

Yang Rui said: "People are only given by cadres at or above the department level, and I can't use Li Zhangzhen's identity certificate." ”

Yang Rui has obviously tried. The staff of the nursing home insisted on their opinions, and Yang Rui did not force it.

After the tourism resources are fully developed, there is nothing rare in such hot springs, it is nothing more than to draw out a pool of hot spring water, more water supply and drainage pipes, you can charge a few hundred yuan more room fees, and stimulate countless hot spring resorts to devote themselves to this.

However, in the current state of resource development, hot springs themselves are not uncommon, but the construction cost has become a bottleneck. In addition, the sanatoriums that develop hot springs are all fully funded by the government, and there is no urge to make a profit, and after the construction of one is completed, there is almost no new investment except for tinkering on weekdays.

A small courtyard with a built-in hot spring is a very fresh experience in Tengchong. In the case of scarcity, it is natural to keep it carefully to avoid the sudden arrival of senior leaders.

Liu Shan tightened the small bag on her chest and said, "There's no one in it, let's sneak it in." ”

Then, Liu Shan took the lead, bypassing the lights at the intersection and picking up the low wall behind.

The poor family's sanatorium for the old cadres didn't take into account that some residents could climb the wall.

Yang Rui looked at it below for a few seconds, and as soon as he gritted his teeth, "What are we afraid of", he followed and flipped in.

Flipping into the small courtyard, Yang Rui was stunned.

I saw that under the bright moonlight, Liu Shan was stretching her waist and taking off her blouse.

In the dimness, Yang Rui saw beautiful curves, and heard small sounds in his ears.

…… (To be continued.) If you like this work, you are welcome to come to Biquge www.biquge.info to vote for recommendations and monthly passes, your support is my biggest motivation. For mobile phone users, please go to M to read. )