Su Xiankai and Fan Zhen were doing their own experiments, their eyes sweeping over Yang Rui's table from time to time.

Since entering the "ion channel laboratory", this is the first time they have seen Yang Rui doing experiments.

Although it is said that the person in charge of the laboratory does not have to have practical ability, just as an officer does not necessarily have to be very strong in individual combat ability, but an officer with strong individual combat ability is obviously more popular with soldiers.

Moreover, Yang Rui is really younger, just like a young officer, always prone to provocation by soldiers.

Su Xiankai and Fan Zhenlong are still newcomers, and they were recruited by Yang Rui, so they didn't dare to challenge Yang Rui's authority, but secretly observed, it was still no problem.

As they observed, both of them also clearly showed disappointed looks.

Yang Rui's operation level is too ordinary.

Of course, this is a judgment made with high standards. Yang Rui has actually improved a lot from the previous experiments, however, since the day he became the head of the laboratory, everyone's expectations for him have also increased.

For example, Xu Zhengping, who is a middle-aged uncle, still does not have his own independent laboratory, but his experimental level has been trained for more than 20 years, although many movements are not standard enough because they are incorrect when learning, but twenty or thirty years of continuous experiments, non-standard actions, also make Xu Zhengping's experimental operation smooth.

On the other hand, Yang Rui, even if some actions are very standard, he still can't hide the jerkiness under his hands.

"Slow. ”

"Too slow. ”

This is the common idea of Su Xiankai and Fan Zhenlong.

As for Xu Zhengping, he had long been accustomed to Yang Rui's experimental actions that were not fast enough, and this time, what he didn't believe was Yang Rui's experimental plan.

What are you kidding about spending a few days doing DNA sequencing?

Gene sequencing technology began in 77 years, when there was a method for determining DNA sequences by chemical degradation, and the dideoxy chain termination method that Yang Rui is doing now, but no matter which method it is, it is not easy to do DNA sequencing with such technology, the error rate is very high, and the efficiency is very low.

Of course, no matter how low the efficiency is, it is stronger than the small fragment sequencer method invented in 65, which is like the potassium ion channel project made by Yang Rui.

Xu Zhengping believes that in those top laboratories, some researchers who are super familiar with DNA sequencing may indeed make DNA sequencing of plasmids in a few days, however, the ion channel laboratory is not a top laboratory, it is not even a provincial laboratory, and Yang Rui is not a researcher who is super familiar with DNA sequencing......

In fact, there are no researchers in China who are super familiar with DNA sequencing.

Gene sequencing is indeed a very important biological experimental tool, and if the "human gene sequencing" project is the peak of biology in the 20th century, gene sequencing can be called the most important experimental tool in the history of biology.

But throughout the 20th century, China has always been slow to beat the crowd.

In 65 years, Sanger invented the small fragment sequence determination method and completed the determination of 120 nucleotides of E. coli 5strna.

In 77, the practical-level DNA sequencing method appeared, and China was busy reprinting books to recruit teachers, so naturally it was too late to learn.

As an associate professor at Peking University, Xu Zhengping came into contact with DNA sequencing a few years ago, and he did a complete experiment in total, which can be regarded as experiencing the most important technology of modern molecular biology. He probably won't do it a second time until his project involves gene sequencing.

In order to save resources, graduate students learn these things theoretically.

Xu Zhengping also knew with his cerebellum that even if Yang Rui had done DNA sequencing, it would be half a time.

Looking at his movements, I also know that he is unfamiliar, and with such skills, it takes a few days to make DNA sequencing.

Xu Zhengping deeply thinks that Yang Rui is losing, let alone relying on this to get floating funds, these reagents that cost a lot of money are all blind.

Yang Rui himself didn't notice the gaze of the people around him at all.

This experiment is indeed difficult for him, and requires full concentration.

Configure buffers, annealing mixtures, these things can be done by the assistant He Quangui and others, and even centrifugation and ethanol cleaning can also be handed over to the assistant to do, but when it comes to adding the solution, and then running electrophoresis, or heating, and cooling on ice, it is all Yang Rui's work.

When Yang Rui was studying, the automatic sequencers used for DNA sequencing were all in the second generation, and the automatic sequencer could not be said to be fully automatic, but there were many more manual steps that could be replaced.

Before the government shut down, ordinary mothers could undergo genetic sequencing and various prenatal screenings.

But in 1984, the current DNA sequencing technology did not even have the first generation, and the United States may not be able to make the first generation of DNA sequencers until next year, and if the Chinese can afford it, it will take another two years - just like the Apple mobile phone, you can afford the money, and you still have to wait for the factory to produce.

Without an automated DNA sequencer, manual work is complicated.

In the final analysis, DNA sequencing is to analyze the base sequence of DNA fragments, adenine is A, thymine is T, cytosine is C, guanine is G, these four letters cycle in various forms, which constitutes the basis of the organism, and DNA sequencing is to measure the order of these four bases.

The principle is simple, but it is not easy to do.

Because the base is invisible, to sequence, you have to make it react, and it's the right reaction.

Accurately reacting the required bases, step by step, is also the devil's step for theology.

Yang Rui's pace was very slow, so slow that Xu Zhengping couldn't stand it, but a few hours later, Yang Rui's electrophoresis was finished, and a clear gel appeared in front of several people.

"Strange and beautiful ......" Fan Zhenlong is older and more social, and he wants to slap Yang Rui on the back, but he finally found an opportunity, although the shot is relatively light, it is finally a sycophant, and he also gained Yang Rui's smile.

Su Xiankai's reaction was a little sluggish, and he followed up with a sentence: "It's very beautiful......

No matter how young Yang Rui is, when he is the person in charge of the laboratory, the lifeblood of everyone in the laboratory is in Yang Rui's hands. From funding to publishing papers, everyone has to be constrained by Yang Rui, and when you meet a better person in charge of the laboratory, you can have more funds, fewer boring tasks, and less trouble when the paper is published. When encountering laboratory leaders with poor character, not only do they have less funds and more requirements for results, but even worse, some laboratory leaders not only want to be the corresponding author or even the first author, but they also dare to give the author's signature to people outside the laboratory to do favors or even pure transactions.

When Su Xiankai and Fan Zhenlong first arrived, they couldn't figure out Yang Rui's temper, but at least they knew that it was always right to follow Mao.

Yang Rui recorded the results and continued to repeat the process just now.

This time, it was another few hours, still getting a beautiful gel, and another round of praise.

Yang Rui repeated it again, over and over again.

From morning to evening, Yang Rui did not stop except for eating, and in the afternoon, he even added a group of experiments.

Su Xiankai and the others had nothing to praise, but Xu Zhengping's eyes were straight.

I got accurate results directly every time!

In the age of manual experimentation, this is very difficult.

In any case, this is also a new DNA sequence of fresh**. If it is a repeat experiment, it is much simpler to start over according to other people's answers, just like the repeated DNA sequencing done by Fan Zhenlong and Su Xiankai, but the new DNA sequencing will always have some more unwanted answers.

Just the last step of running electrophoresis often encounters the situation that you can't run out, and then you fall short.

Yang Rui was slow at every step, but he didn't encounter a mistake, which made Xu Zhengping almost dare not think about it.

But the unthinkable scene appeared in front of his eyes now.

It's like a final exam, only one student scored 100 points, you say he plagiarized, this is not right - if it is really an exam, then there is still the right answer, Yang Rui is doing now, but the world's only Tengchong thermophilic s5 fragment sequencing, not to mention that foreign researchers do not have Tengchong thermophiles, even if there is, they have not sequenced and published public papers, this, Xu Zhengping, who often checks the information, is very convinced.

"It's beautifully done. At eight o'clock in the evening, watching Yang Rui make another version of the clear gel, Xu Zhengping praised it from the bottom of his heart.

Yang Rui smiled and said, "It's just an oil seller." ”

"You're a groundbreaking experiment, and it's useless to just get acquainted with it. Xu Zhengping didn't believe him.

Yang Rui actually said that he missed his mouth, he followed the answer in his mind, and speculated on the order, of course, it was accurate every time, so he naturally would not refute at this time, pretending to be modest and smiling again, and said: "Do another group, I will rest, if you want to go back, go back first." ”

"There's no hurry, let's all take a look and learn. Fan Zhenlong made another slap in the face.

"You all have families and rooms, why don't you go back to accompany your wife and see what the point is in me doing experiments. ”

"Moral support. Fan Zhenlong's answer was short and forceful.

"I'll give you a hand, my experiments are done, and the reagents I prepared today have all been used up. Su Xiankai is more direct.

Many reagents, such as buffers, are ready to be used, and generally speaking, they are configured when they come in the morning, and how much to allocate according to the progress is decided, and the experiment should be finished when they are used up. Of course, some demanding experiments can't even be stored for a few hours, so they can only be rematched occasionally.

Su Xiankai has already prepared more reagents, but he is not ready to do it at eight o'clock in the evening, and now he wants to continue to do his own experiments, but he has nothing to do.

Yang Rui gave him a place and said, "Then stay with me for another two hours, I'll finish this sequencing in the next few days, and it's good to get tens of thousands of dollars more, right." ”

"Of course, tens of thousands of yuan is good, but it's really enough to catch up in a few days. Su Xiankai told the truth.

Yang Rui smiled and said, "I'm a little tired, but I should be able to finish it." ”

Su Xiankai couldn't help but say: "The S5 fragment of Tengchong thermophilic bacteria has a total of 126 base pairs. ”

126 base pairs, which is relatively short. Yang Rui made 5 in one day, which is also extremely fast, but he also knows that he can't complete this project in a few days with elementary school mathematics.

In fact, this has quickly taken off, in foreign countries, many laboratories sequence a gene strand, and there are tests for one or two years, and all kinds of strange problems encountered during the period are difficult to write.

Yang Rui did it so smoothly today, which is already amazing, but to say that it is smoother and faster, Su Xiankai really can't shoot such an excessive sycophant.

Yang Rui didn't have to explain, just continued to do it.

No one noticed that his proficiency had increased now.

……