Chapter 412: Change

"Yang Rui, if you don't come back, the scholarship will be swallowed up. Bai Ling tried her best to make her tone appear urgent, but on the phone, it was still too soft.

Yang Rui shook his head that was a little wrong in trial and error, and asked, "What scholarship?"

Bai Ling couldn't wait to repeat the "coup d'état" that happened not long ago, and said: "What are you doing, at least show your face in class." ”

Yang Rui was more amused than angry, and said: "Forget it, if he wants it, give it to him, I didn't want a scholarship in the first place." ”

"Even if you don't get a first-class scholarship, it won't be Geng Jian's turn. Bai Ling took a look and said: "Geng Jian is going to find a counselor now, if you don't show up again, he may turn his head and go to the department to complain, maybe he wants to say something about you, you should take the time to come to the school to have a look." ”

Yang Rui was really tired after doing it for a few days, and he thought about it and said, "I'll arrange my work and go back to school on Monday." ”

The current work of the Huarui real yàn room is to keep trial and error, and at the same time, it is also to keep reproducing fruit flies with genetic mutations, Yang Rui has found some means to save time as much as possible, but it is indispensable to try again and again.

Huang Mao, as a future bull-level player, has much better intuition than Yang Rui in terms of real yàn, and during Yang Rui's absence, he has also completed about 3% of the progress, which can be said to be quite excellent.

In fact, in addition to being slightly better in directionality, the current Yang Rui is not confident that he can win against Huang Mao.

However, the work of the owner of the real yàn room is more directional.

Yang Rui provided the direction of the real yàn, decided the real yàn scheme, and proved the real yàn result, even if he did not participate in the real yàn to a large extent, as the first author of the paper, no one doubted.

In the two days of the weekend, Yang Rui re-optimized the practical plan, and after verifying it, he handed it to Huang Mao again, saying: "I'll go back to school, you guys work harder to see if you can speed up the progress." ”

"I'll try my best, to be honest, the speed of research has reached the limit, as I said before, the chances of getting a paper before April are really not high, and the time is too tight...... Hmm, wait, it's a bit interesting......" Huang Mao glanced up suddenly, and his eyes were once again focused on the practical plan provided by Yang Rui.

Yang Rui sat on the chair with peace of mind, smiled slightly, and didn't say too much.

To study the role of a gene, it is necessary to induce mutations in this gene, and then to see which behaviors of the organism or biological system have changed, and then the role of the gene can be determined.

It's actually a lot like fixing a computer. In the case of two computers, one computer has a problem, the hardware of the computer with the problem is taken off one by one, and replaced with the hardware of another computer that is determined to be normal, so try one by one, when it comes to which hardware is replaced, the computer can not work normally, and it is natural to know whether there is a problem with the memory, or a problem with the motherboard, or a hard disk, cphj... ̈

Unlike repairing computers, organisms have many genetic functions, and it is difficult to test them one by one.

Sometimes, researchers have to devise some ingenious or tortuous way to detect two or three, or five or six factors at the same time, and finally mathematically determine the problem of one or several of them.

These ingenious methods did not fall from the sky, but were developed by researchers from generation to generation, trying and even by mistake.

When Yang Rui was in graduate school, many methods had become basic skills.

But at the moment in 1984, Yang Rui has become the pioneer of the cloning mutant gene method, and the method that he was once familiar with, or unfamiliar with but easy to use, has naturally not been invented.

In recent days, Yang Rui has also been working on the technology to be used in practice.

The technology is not the more advanced the better, first of all, the equipment of the room must be cooperated, 26 years later, it is normal to give a few students an 86 rpm 1060 rpm centrifuge, 84 years of domestic colleges and universities do not have such conditions.

Not only should the hardware requirements be low, but the software requirements should not be too high.

Several people in Hua Rui's room, except for Huang Mao, Tu Xian, Wang Xiaoyun and Wei Zhenxue's practical level are relatively average, and they belong to a category of people who are less than the top and more than the bottom, and there are many skills that pay attention to skills, so it is very tiring for them to operate.

A set of suitable techniques is not only one step, if a series of difficulties are only 8% of the spoon correct, then the seven difficulties, the final accuracy is only 2%.

In biology, the high technical difficulty is really high. For example, cloning, the technology of cloning is basically public, but the real room that can clone sheep, clone pigs, and clone rabbits can be found all over the world, and the number of real rooms that can be done comes out, of course, many real rooms that are not done are not necessarily impossible to do, maybe they are just afraid of difficult action requirements, complex real steps, and the costs and benefits that outweigh the losses.

Now that we are not long away from the cut-off point, we must not only pick the ones with low hardware requirements and simple operating conditions, but also pick the ones that learn quickly or do not need to learn.

Under so many restrictions, Yang Rui has not many options.

Oligonucleotide-induced gene mutation technology is one of the site-directed mutation technologies that have developed rapidly since the end of the 8s.

This technology was invented in / year, but in the early 8s, it was in the perfect period, and Huang Mao also came into contact with it, but it was not commonly used, because although the ogonucleotide-induced gene mutation technology greatly increased the accuracy of mutations, in practical application, the incomplete synthesis in vitro, the destruction of mutant primers by in vitro reactions, etc., all made this technology troublesome.

In response to these troubles, Yang Rui clearly gave a solution with a short narrative.

First, nucleosidase digestion; second, alkaline sucrose gradient ultracentrifugation; the third is gel electrophoresis under the condition that ethidium bromide is stored in zài, and the fourth is cesium chloride density gradient centrifugation; The fifth, hydroxyapatite adsorption single chain.

Seeing this explanatory essay that was written in a very specific way, with something to say, and put forward five solutions in one breath, Huang Mao's head "buzzed", as if a hole had been opened in the door of his brain, and the rain poured into it, stirring and stirring in his brain, making one idea burst out, created, disappeared, and created again.

After about ten minutes, Huang Mao read the plan again and again, sweating on his head, and said, "How did you come up with these methods? You didn't stay in the real room for a few days." ”

"I'm going home for the New Year, and I'm not idle. "Yang Rui is indeed not idle, but he is doing the real yàn of coenzyme QL0.

Huang Mao didn't doubt him, nodded and shook his head, and said: "It's normal that you can study these methods, but I didn't expect you to study it so carefully, okay, I'll give it a try with your plan, and our progress is just stuck here." ”

Yang Rui smiled and thought: Of course it's just right, I just saw that you were stuck here, so I provided you with this technical solution.

After accompanying Huang Mao and others to practice for two days, and settling down in the real room, Yang Rui went back to school.

In the first class on Monday, Yang Rui almost stepped on the class bell and rushed into the classroom.

A room full of students looked at Yang Rui with different expressions.

If it were a different person, the students might not be able to recognize it, but Yang Rui was too conspicuous. Although he doesn't wear bright Adidas now, even if it is an ordinary turtleneck sweater, put on a high-value player like Yang Rui, it can show a difference.

What's more, in the past two days, Geng Jian has been able to vigorously promote Yang Rui's privileges.

The teacher on the podium also knew Yang Rui, smiled gently, and said, "Come early next time." ”

Turning his head, he began to lecture.

Four classes were arranged on Monday morning, Yang Rui complained about too many classes, while looking for information in his mind, he is now used to arranging his time extremely full, and it rarely happens to him when he is idle or something.

When the bell rang for the lunch break, Geng Jian stepped onto the podium vigorously and said, "Students, please wait a minute, we have to vote and score." ”

The teacher was still on the podium, glanced at Geng Jian, and said, "Students with problems come to my office, and now the class is over." ”

The teacher left, and the students packed their things and waited to hear the arrangement.

Yang Rui looked at the classroom full of people, and couldn't help but sigh: The students of the 8 years are just well-behaved, and after 3 years, where can they wait obediently in the classroom and be quiet.

Geng Jian stood in the center of the podium, coughed vigorously, and said: "Today's main thing is to evaluate the moral education score, the specific situation, everyone already knows, students who don't know, ask yourself." As for me, I also talked to the counselor, our scholarship evaluation this time, moral education scores accounted for 3, final exams accounted for 6, other honors accounted for 10, comprehensive evaluation from high to low, each class is a first-class scholarship, two second-class scholarships, and four third-class scholarships. In addition, I would like to explain the composition of moral education scores, moral education scores mainly look at everyone's usual table, among them, sports scores account for 10 moral education scores, social activities account for 40 moral education scores, attendance accounts for 6 moral education scores... Yang Rui, if your attendance is calculated, you will get 0 points. ”

In the last sentence, Geng Jian pretended to be concerned and attacked Yang Rui in this way.

Yang Rui was stunned for a moment, then nodded and said, "I know." ”

Bai Ling was in a hurry, didn't care about avoiding suspicion, stood up from a group of girls, and said: "Yang Rui, don't be provoked by him, you are asking for leave to go to the real room, and you are not running out to play, why should you deduct attendance." ”

Nowadays, boys and girls still sit in groups, and it is rare for a girl to squeeze into a group of boys, or a boy to squeeze into a group of girls.

Bai Ling helped Yang Rui speak, which immediately attracted a soft laugh, but Bai Ling didn't care.

Seeing this, Geng Jian felt all kinds of unhappiness in his heart, and he still suppressed his anger, and said: "If you don't come, you don't come, and you don't come when you ask for leave." If he hadn't asked for leave, he would have been dissuaded from the school by not coming to so many classes. ”

Bai Ling wanted to argue for Yang Rui again, Yang Rui smiled, stood up and said, "It's okay, I really didn't come to class, I should deduct points, otherwise it will be unfair to other students." Geng Jian, just do things according to the rules. ”

Bai Ling was stunned for a moment, but she reacted quickly and sat down again.

Geng Jian prepared a basket of words, and suddenly there was no use.

Bai Ling held the back of the chair with one hand and looked at Yang Rui quietly, one was competitive, the other was light and light, and the judgment was high.

As for scholarships and stuff, it really doesn't matter.

"I haven't been able to come to school these days because the work of the real yàn room is at ......a critical juncture," Yang Rui's voice sounded again, but he explained very seriously: "I may have affected some classmates by doing this, I should say sorry for this, don't learn from me." ”

Soft laughter rang out in the classroom.

"Exactly. Yang Rui clapped his hands again and said: "The foreign factory I contacted sent a batch of ham sausages, let me find someone to help taste it, and those who see it have a share, one by one ham sausages, after eating, tell me if it is delicious, where it is delicious, where it is not delicious, just write it." ”

As soon as Yang Rui's words fell, the Jielikang employees who had been waiting at the door sent two baskets of ham sausages in. There is also a stack of paper on each box.

There were two major students in the classroom, more than 100 people, looking at the two pointed bamboo baskets, all of them a little stupid.

Ham sausage is much more expensive than meat, and it is given away for nothing? Foreigners are stupid?

"One person has a ham sausage, one person has an evaluation form, you're welcome. Yang Rui grabbed a box and distributed them one by one.

This is really a sample sent by a foreign factory for marketing, Yang Rui asked Jielikang to buy some ham sausage, and the other party immediately found an affiliated company and gave him free of charge.

Of course, if it weren't for Yang Rui's call, ten out of ten of these marketing samples would have to go into officialdom marketing.

These intercepted by Yang Rui really deserve the evaluation form.

The students who first took the ham sausage were a little embarrassed, but after a little more people, the people behind them took it naturally.

Some students were reluctant to eat immediately, and asked loudly, "Is it okay to hand in the back of the evaluation form?"

Yang Rui immediately agreed: "Just give it to me within three days, if I don't have it, let me go in the dormitory." ”

"Okay" There was a burst of laughter in the classroom, and almost no one remembered what was happening in front of them.

After the two baskets of ham sausages were distributed, the students' perception of Yang Rui changed greatly. They are all fresh** college freshmen, there is no grudge or grudge, and they naturally decide their preferences based on their personal senses.

Being able to go to the real yàn room to do things, that's people's ability, and being able to remember to bring ham sausages back to everyone is the true love

When Yang Rui returned to the dormitory building, he came and went, and people kept greeting him, and some people came over to bump his shoulders and pat his arms.

In the girls' dormitory, everyone also actively talked about Yang Rui.

"You say, study well, don't cause trouble, and write papers, I can understand this, he gave everyone ham sausages, so many ham sausages, foreign companies, really give it to him?" Bai Ling's lower bunk, Zhao Yan, bit the slightly salty casing, and couldn't help but be curious.

The eldest sister in the dormitory has a child, so she wrapped the ham sausage with a cloth and put it in the cabinet, saying: "The ham sausage made by our domestic factory is made of scraps, and the foreign imported ones are different. ”

"Then how did you give it to him. ”

"People have the ability. Bai Ling, did you say?"

Bai Ling said "ah", pretending to be casual: "I admit that I have the ability, but what I said doesn't count." ”

"I have the final say. Yang Rui is really capable. The dormitory sister smiled and said, "Do you know how much a ham sausage costs in the friendship store?"

"More money?"

"2 yuan a piece. ”

"How much do those two baskets of ham sausages cost?" said the girls in the dormitory, as if listening to a story of surprise.

"It's not a question of how much money you have, the ham sausage in the friendship store is not something you can buy if you want to. Therefore, you have to have some skill to buy two baskets of ham sausages, and how much ability does it take to let a foreign company give you two baskets?"

The women seemed to have a sudden realization.

……