Yang Rui was silently planning for his PcR project, and the assistants around him only saw him reading literature, reading literature in a daze, doing experiments by himself, and did not know what Yang Rui was doing.

At this point, Yang Rui already looks like a normal laboratory leader.

In fact, Yang Rui's work in the past six months has been all about preparing for PcR.

First of all, there is the ever-expanding scale of Sinovel Labs. PcR stands for polymerase chain reaction, and it is a molecular biology technique.

In 1984, as long as anything is associated with "molecules", it is like "nano" in the 21st century, and it has entered the high-tech field.

High is high at high cost, and new is new with new instruments.

Since the construction of Sinovel Laboratory, the cost of equipment has exceeded 1.5 million US dollars, which is Yang Rui's dividend for more than two quarters, which is equivalent to spending one-third of Yang Rui's income.

If you change a rich person here, you will be scared to pee out when you see such an expenditure.

This is a pure investment, and it is very mysterious whether it can be recovered. Although Yang Rui has proven himself once, it is still difficult to say whether he will win if he falls into the second scientific research competition.

Fortunately, there is no boss above Yang Rui, which allows him to prepare for PcR freely, otherwise, don't look at Academician Cai of Peking University who values him so much, but if Yang Rui says that he wants to buy equipment with $1 million, it is also free to talk.

In the scientific research community, if you have a low status, you have to do a project with few resources, otherwise, you can't do it, and without the life of the princess, don't expect the tutor and the principal to treat you like a father, the princess's disease is a terminal disease, even if it can't be cured, try to control it and improve the quality of life, it is still necessary.

In addition to the soft and hard conditions of Sinovel Laboratory, Yang Rui went out of Tengchong and then made high-temperature polymerase, which was also prepared for the PcR project.

But there was no need to tell anyone about these preparations.

Even Huang Mao, who personally made high-temperature polymerase, didn't know what the high-temperature polymerase was for.

This is a situation that people who do research often encounter, anyway, if it is made, it is even a result.

is also because he is not in a hurry to use it, Yang Qi asked Huang Mao to slow down the ** text, and he agreed without hesitation.

And below the high-temperature polymerase, it is a serious PcR.

PcR can be complex or simple. Complex PcR is the simplest for the user, the so-called "fully automatic PcR", 20 years later, the students are almost using this, just throw the material in, press two buttons on the machine, and then wait for the thing to be spit out.

Simple PcR is complicated for users, each cycle must be supplemented with polymerase, and the reason for supplementing polymerase is that each cycle of PcR has a process of denaturation, through high temperature means, so that the double strands of DNA are unraveled, which is a prerequisite for replication.

Before the discovery of high-temperature polymerase, the general polymerase could only withstand a temperature of 37 degrees, and the DNA unraveled the double strand, and the polymerase was finished.

Normal DNA is made up of two strands entwined together, and the process of copying the two strands separately with polymerase and putting them together is the process of replicating DNA.

In this case, DNA is like a girl covered in white phosphorus and wearing a safety suit.

The polymerase enzyme responsible for replication is like a man who has lost his essence.

The process of replicating DNA requires mating.

And if a man wants to mate, he must first tear off the safety suit.

Tearing off the safety suit, the white phosphorus with a low ignition point burned instantly, and the man with a low heat resistance level was burned to death, and another man needed to be added later, because the white phosphorus had been burned out, and this time the replication was successful.

However, this is only the first set of loops.

When the mating man enters the second cycle, he is obviously about to be burned to death by the brand new burning chastity pants, so a third man joins in.

There are drawbacks to the constant addition of men, first of all, the corpse is more difficult to handle, the look and smell are not good, which affects the subsequent mating, accumulates too much, and even squeezes the space.

Therefore, the best thing to do is to find a man who can withstand high temperatures, and the "burning chastity pants" will appear in a cycle and will not be affected.

For a practical PcR, this technology can only be of practical value if you find a man who can withstand high temperatures.

Muris's PcR suffers in this regard.

Yang Rui can save half a year just by avoiding this one, but he also has a waste of time.

He didn't have the right piece of genes in his hands.

To be precise, in Yang Rui's hands, there is no sequence analysis of gene fragments.

Human genome sequencing refers to the sequence analysis of human gene fragments one by one.

Because in order to copy a gene fragment, you must first know what is in the gene fragment, that is, to know all the information about the girl in the safety suit, has she ever learned judo, how tall is she, and how strong is she?

The sequence analysis of the gene fragment is determined, the primers can be determined, and then the appropriate polymerase is sent to replicate, for example, when you meet a judo girl, you send Putin, not Abe, although the latter has something to do with judo, but it is not a proper match, and it will still be strangled without hesitation.

When Morris was doing PcR, he was in a large company, and people were doing their own work of cloning gene fragments, so naturally there were various gene fragments to analyze.

Yang Rui does not have this condition, and even in China, Yang Rui has to find suitable genes in published papers.

This job is easier said than done, mainly because I am afraid of encountering paper fraud, or stupid papers written by fools.

The so-called not afraid of 10,000, just in case, there are many such things as paper fraud or wrong papers, China is not new, the United States is not new, and neighboring Japan is also exposed every year.

If it is an ordinary paper, Yang Rui will not delve into the authenticity of other people's papers, and it can almost be used.

But PcR is a $3 billion project, and if it succeeds, there is no telling how many pharmaceutical and biotech companies will be watching, and Yang Rui can't let his paper be built on an unstable paper.

Papers that are not made by laboratories that are closely connected with oneself, and if they are not verified, if there is any problem at that time, it will be a gap to attract flies for a $3 billion project.

If it is a gene fragment analyzed by his own company, Yang Rui can also review the experimental process, and if it is a gene fragment analyzed in China, Yang Rui can also ask Academician Cai - the other party is willing to show the best, if he is unwilling, he can also have a minimum judgment.

However, Sinovel Labs does not have such reserves.

Then you have to repeat the experiment yourself.

However, the work of gene sequence analysis can be handed over to the "ion channel laboratory" of Peking University.

Because according to Yang Rui's design, genetic analysis was originally one of the main goals of the official laboratory.

The future "Human Genome Project" is a huge project costing hundreds of billions of dollars, and the genetic sequences obtained by the Human Genome Project are all open to the public and cannot be profited.

No matter how rich Yang Rui is, he can only pretend to be pure in the face of this kind of plan.

Of course, genes other than the "Human Genome Project" can still be profitable, such as the genes of Tyrannosaurus rex, you can find something to study, and no one cares about you.

But in any case, these are plans for the future, and for now, repeating the sequence of several gene fragments is purely basic research, and it is also a good way to train troops by leaving it to the "ion channel laboratory".

After carefully selecting a few plasmid fragments, Yang Rui immediately opened several small projects for cloning target fragment sequences in the ion channel laboratory.

The newcomers, Su Xiankai and Fan Zhenlong, were eagerly looking forward to proving themselves, and it was not surprising that they took over the job.

Xu Zhengping was a little strange that Yang Rui repeated the experiment so much, but he had a lot of work under his hands, and he couldn't take care of others.

The only problem is that there are not enough experimental dogs.

Su Xiankai did it for more than a day, so he couldn't hold it back, found Yang Rui, and said: "Professor Xu has the help of his students, the public assistants of the laboratory, He Quangui and Zhao Pingchuan don't come every day, and they also have some regular jobs, and I need at least one special assistant to do experiments." ”

"This is as it should be, I will arrange someone to help you first, you can make a report, and I will go to Professor Cai to ask for someone." "Yang Rui's laboratory is also operational, and the two researchers who are in the compilation are in place, and it is time to recruit experimental dogs.

"I want it too. Fan Zhenlong raised his hand at the other end of the lab.

"One person, one full-time assistant, I know. Although Yang Rui brought the establishment of the two to Peking University, it would take a long time for the school to arrange teaching positions for them, and during this period, they naturally had no way to find the experimental dog by themselves, so they could only be arranged by Yang Rui.

However, seeing that his independent laboratory was up, Yang Rui also had a different mind.

He deliberately asked the two of them to make a report, found a time for Academician Cai to be in the office, and rushed over, handed in the report, and said: "In order to do research on coenzyme Q10, with the help of Jielikang, I got a joint laboratory with Hedong University in Hedong Province, called Jielikang Hedong University Laboratory. There are a lot of students in the laboratory, all of whom are carefully selected from the biology department of Hedong University, and many of them have been doing experiments for two or three years. ”

What Yang Rui has to do is naturally transfer Yao Yue over.

Academician Cai didn't know why, and said strangely: "Can they agree to transfer people from Hedong University? Teachers or students?"

"I want two students who have been doing experiments for a long time, so that they can get started when they come and don't have to train anymore. The students in our school who will do experiments are all masters, and the rest are either not interested in this, or they have to be carefully selected...... On the other hand, I think that Fan Zhenlong and Su Xiankai are both transferred from other schools, and the senior students may not be willing to cooperate with them when they first arrive, and I am familiar with the students in the laboratory of Jielikang Hedong University. Yang Rui has a lot of reasons.

Academician Cai groaned: "There is no such precedent before. ”

Yang Rui smiled: "It's only been a few years since the college entrance examination was resumed, there is a precedent for you to do it, who dares to make irresponsible remarks." Isn't there such an exchange student abroad? Let's exchange two in one direction, and Hedong University should not object to it. ”

This matter is a little troublesome to talk about, and it is impossible for Academician Cai to agree to it when ordinary teachers bring it up. However, the status of the "ion channel laboratory" in Academician Cai's mind does not stop there, and the cooperation problem raised by Yang Rui made Academician Cai a little concerned.

Today's college students are still very arrogant, Peking University's students are naturally even more arrogant, Fan Zhenlong and Su Xiankai were selected by Yang Rui to be transferred, they are not very old, and they do not have particularly good results, if they meet two Peking University students who can't speak, they may delay the progress of the experiment.

The harmony of the laboratory cannot be seen or touched, but it cannot be ignored.

Thinking of this, Academician Cai asked, "How do you want to operate?"

"Is it okay to ask Jielikang to come forward? Send two students from Jielikang Hedong Laboratory to Peking University for one to two years of exchange, and there is no need to change the student status, let's arrange it here, treat it as our own students, we should attend classes, we should have meetings, we should focus on the laboratory, and then let Jielikang give a little tuition and miscellaneous fees or something......"

"What if you're not satisfied?"

"Then just call back. ”

"Well, we need to get the consent of the student and the student's parents, and write a written consent form. ”

"No problem, come to Peking University, what else is not happy. Yang Rui said that Academician Cai touched his beard and laughed.

……