At this time, Bai Liyan rushed into the study, spread out the four treasures of the study, and wrote two express letters in a row, one was sent to the royal guest, and the other was sent to Du Qi.

During this period, Zhou Kong and others knocked on the door repeatedly to ask despite the dissuasion, but they were all driven away by Bailiyan. Half an hour later, the women and their subordinates, under the protection of the male and female guards, left the house in a big car and went to the south of the city. It wasn't until half an hour had passed that Figos arrived at the Hou Mansion.

It is said that Gudappa's mansion is only a quarter of an hour's walk away from his Yongxing Marquis's mansion, and this half-hour walk is too much. Now that the situation is urgent, Bai Liyan couldn't help but be a little annoyed and asked him:

"Why did you come, your father-in-law?"

"I was invited to the banquet by the dead fat man, and I was just dragged by your people from the banquet."

Figos was quite reasonable, and Bailiyan then said:

"So, you're going to Baicui Building?"

"No, or I would have been able to walk for half an hour."

From the Baicui Building to the Hou Mansion in fact, it will not take half an hour, after dark, the curfew will not be implemented in the Lingling, and the crowds on the street will be surging, so in principle, do not let the horse ride and not let the car, if you don't come sincerely, wander around while walking, it is not impossible to walk for an hour on the road.

"Okay, there's something for your father-in-law to do immediately, and tomorrow."

"What's so urgent?"

"Do you have pimples on your cattle farm now?"

"Yes, what are you asking about?"

"Tomorrow all the pimpled cattle will be picked out, and the others will be raised, and my men will remove the cattle within three days, and I will replenish the cows you have lost as soon as possible."

Figges felt strange and asked:

"What do you want to do with a cow with pimples, if this disease starts, it will be transmitted to people."

"I know, but this kind of pimple is not fatal, at most it will be fine for a while, leaving a few pockmarks on the hands."

At present, cowpox is called a pimple, and cowpox is actually a smallpox virus in cattle, but cowpox is not fatal because of this, it is only equivalent to a kind of pimple that does not hurt or itch. People can be infected with the cowpox virus, especially the milker, whose hands often come into contact with the cow's **, so the milkman is infected with cowpox is a high incidence of disease, but the cowpox will not cause the malignant effect of smallpox infection in humans.

Of course, if you are born with a weakened immune system, bovine smallpox can be fatal. At the same time, after bovine smallpox was planted into the human body, it naturally formed human smallpox immunity, so there was cowpox.

When Bai Liyan was 10 years old, she did not know that there was no smallpox in the Central Plains, and because she was worried about contracting smallpox, she infected her wound with cowpox and gained immunity.

He is not worried about smallpox now, but about whether the cowpox virus on the planet will be able to develop immune antibodies to the smallpox virus after it infects the human body. If not, it's quite tricky.

That night, Bailiyan bathed and changed clothes, and burned the clothes she was wearing today. The next morning, when the court was over, the cabinet meeting was dispersed, and Bai Liyan did not leave, so the king of Xian asked him:

"Yongxing Hou must still have private affairs to report privately."

"King Shengming, last night the minister settled his family in another courtyard, I think the king already knows, and the minister left alone to report this matter to the king."

Sweep all the family members out of the house, it is impossible for such a big movement not to alarm the ears and eyes of all parties, King Xian knew the news last night, and did not send anyone to ask, just waiting for Bailiyan to come today to confess and give an explanation.

"Didn't Aiqing think about marrying Mrs. Nine again, and fell out with all the ladies?"

King Xian asked strangely, and Baili Yan was busy defending:

"No, no, no, the minister has no courage to marry another room, there is another hidden reason that has to be done."

"Since there is a hidden feeling, Aiqing will quickly say that the widow will listen to it, what kind of hidden love can make Yongxing Hou drive away the eight flower-like beauties to keep a lonely night, hehahaha......"

King Xian pricked up his ears with great interest, Bai Liyan knew that King Xian was joking with him, but he couldn't be happy.

"King Qiyu, it's a plague."

He replied truthfully, and King Xian's smile froze in an instant, and he stiffly maintained his mechanical movements and said:

"Yongxing Hou Mo is not joking with the widow!"

"No, two months ago, there was an outbreak of plague in the northwest of Xu State, spread to the Yuke General Hall, the number of infected people in the main hall can increase by more than 2,000 people in January, and seven or eight out of ten of the dead are incurable and send people to ask for help from the minister, the plague spreads all the way to the Central Plains with people and horses, and letters, and the minister touched this letter yesterday, so just in case, the minister has no choice but to let his family move out of the Hou Mansion for resettlement."

"The country of Xu is thousands of miles away, and the plague can spread to our country!" King Xian asked, with an extremely contemptuous attitude.

"King, this disease is different from cholera and other plagues, there is no cure for this disease, the current fatality rate is nearly ninety percent, and those who live are all disabled for life. The minister had to be cautious. ”

King Xian was a little angry, Bailiyan knew that the plague was extremely contagious, and he had to hide it and not report it overnight, and bring it into the palace to harm people. King Xian immediately lowered his face and said:

"Why didn't Aiqing report it last night, and he still ventured into the palace today."

"Back to the king, the disease is transmitted by touching people, as well as touching the saliva of the sick, the body and the night and the clothes and utensils, not through the air, so the king and the ministers are not contagious when they talk to each other. In addition, the minister bathed twice yesterday and this morning, and burned all the clothes and shoes and socks he was wearing, so there was almost no risk of infection, and the king could relax. ”

King Xian naturally didn't understand the principle of the spread of infectious diseases, but he understood that he was a lot older, and he died one day earlier and one day later, and there were many people who were looking forward to his death, but Bailiyan would not expect him to die. The reason is actually very simple, if he dies early, Bailiyan will not get any benefits.

At the next moment, King Xian loosened his tight heartstrings, bent his back as if relieved, leaned on the soft backrest of the seat and said:

"According to Aiqing, there is a way to prevent and control it, but there is no cure, how can a widow control the subjects of the whole country and not let the people touch each other, is it impossible to even have the joy of men and women."

"It's not, there is no cure for this disease, but it is not incurable, in fact, if you don't hide it from the king, the minister will not be infected with this disease."

King Xian was shocked when he heard this, and hurriedly asked:

"Aiqing is not a god, how can she not get this disease."

"This matter is a long story, the minister got a law in his early years can make people eradicate this plague for life, so the minister has made arrangements last night, and there will be results within five days. In addition, the Marquis of Lu also asked the king to replace the ministers with one or two, I am afraid that this month will not be able to meet the dragon and phoenix, in case of illness, the consequences are unimaginable. ”

"Well, no one knows about this matter, Aiqing should do it as soon as possible, and be sure not to let the plague epidemic affect the subjects of my Xian country."

"No!"

After negotiating with King Xian, Bailiyan immediately left the palace and rushed out of the city to take over the infected cows of Gudapa. The people of Jinxuedi have the habit of drinking milk to make ice cream, and Gudapa has been doing it himself in the early days after setting up a cattle farm, and he and his family have been infected with a certain degree of cowpox and are immune to smallpox.

Later in the afternoon of the same day, Du Qi rushed to the house to resume his life. Bai Liyan explained the smallpox incident in detail, and handed over to him the prevention and control measures drafted last night and the process of cowpox culture and inoculation.

After nearly 20 years of construction, Yicaotang has basically perfected conventional disciplines such as cytology, bacterial microbiology, immunology, infectious diseases, pharmacy, pathology, anatomy, medicine and surgery, and agricultural plant science is also classified as medicine. Long-term planned drug research and the development and cultivation of beneficial bacteria have yielded fruitful results in the past 20 years.

Because smallpox is a virus, the virus is orders of magnitude smaller in diameter than cells, and until the advent of electron microscopy, "viruses" could not be observed by conventional light microscopes. Due to the lack of observation methods, Bailiyan has rarely mentioned the virus in Yicaotang's teachings, and the sporadic mention is also due to the inconclusive laboratory tests, and it is presumed to be a virus. On this occasion, the concept of the virus was formally proposed to facilitate the cultivation of Yicaotang.

In view of the fact that cowpox is also a virus, there is no advanced observation equipment, and its purification work has risen more than one step compared with the purification of bacteria and fungi, and there is no technical basis and material conditions for the production of conventional inactivated vaccines.

Collecting vaccinia virus as seedlings and cultivating egg embryos to expand the population can obtain a sufficient amount of vaccinia virus for inoculation faster than simply collecting cow infected cattle.

Previously, Yicaotang's microbial research only stopped at the culture of common low-pathogenic bacteria and beneficial flora, and the risk of disease was almost zero. Considering the virulence and contagiousness of the vaccinia virus, Bailiyan began to consider setting up a separate isolation center in a remote area to cultivate and study infectious diseases.

On the second day after Du Qi's arrival, others arrived in Chengling with collection equipment, and after briefly sorting out the situation, they began to collect virus seedlings, and two days later, the first batch of live vaccinations began.

Because the live vaccine is better than the inactivated vaccine, the immune effect is better and the effect is long-lasting, the effective time is long, and there is no need to repeatedly inject multiple times within the action time, the disadvantage is that the seedlings are easy to multiply with miscellaneous bacteria, and interfere with the reproduction of seedlings, resulting in immune failure, and the reproduction of microorganisms is not conducive to preservation and transportation, so long-term storage requires a low temperature environment of minus 15 degrees.

In view of this, Bailiyan went to Yongxing City to coordinate the production of pressure vessels and liquid carbon dioxide for the preservation of seedlings.

Liquid carbon dioxide is one of the earliest liquefied low-temperature gases in physical chemistry courses in recent years, and it is also the easiest gas to be liquefied by compression, which requires much lower pressure and storage than hydrogen, oxygen, nitrogen and other gases, and the simple compressor driven by a steam engine is easier to produce than liquid nitrogen and liquid helium.

The pressure vessel used in the previous experiment is mainly carbon steel, after forging heat treatment, the upper processing machine is processed into a sealed container with a capacity of two catties, the bottle mouth is equipped with a pressure gauge and a simple pressure relief safety valve for easy access, the bottle is plated with a very thick layer of silver, and the cotton sleeve with a thickness of half an inch is insulated to prevent accidental frostbite during handling. Silver can insulate heat, reflect infrared hot wire, is conducive to heat preservation, and also has a certain corrosion ability.

Today's live vaccine storage requires larger containers, and previously designed bottle-sealed pressure vessels are stretched thin. The production of large quantities of carbon dioxide requires specialized equipment, relying solely on laboratory production, even if the cost is too small, and the development of a new generation of standardized compressors and storage equipment is imminent.