Chapter 1176 Preparation of Penicillin by Soil Method

Orange peel can also be eaten.

In the independent regiment of the Sichuan Army, there are many Sichuan people.

In the recipes of Sichuan people, orange peel dried stew, can also be regarded as a flavoring agent, for the Sichuan army who are far away from home to fight, do not know if there is a chance to return to their hometown, can occasionally eat this taste, can also relieve the pain of lovesickness.

Of course, the orange peel that can be eaten is not eaten directly as food, more because in traditional Chinese medicine, orange peel is a medicine.

For colds and coughs, bronchitis, lowering blood pressure, burns, etc., orange peel has a certain curative effect.

In this era of scarcity of medicines, orange peel is more used as medicine for emergencies, so that it can escape the doom of being eaten in the first place.

Lu Yuan carefully poured the few orange peels on the table and picked them up.

Most of the orange peels have dried hard and have a faint orange fragrance, but some of them are starting to get a little moldy due to improper storage and heavy rain for almost a month a while ago!

More than a dozen or twenty pieces of orange peel with patches of green mold were selected and immediately sealed in glassware by two of Dr. Wang's assistants.

"The cyan plaque on this is penicillin!" The road is far away.

"It's that simple?"

Wang Ma Gong and the others screamed strangely: "Then why are you still pretending?" Wouldn't it be enough to scrape it off and give it to those seriously wounded? ”

"If it were that simple, I would have done it a long time ago, and I would still need you to be here? Do you still need Dr. Wang, who have been studying hard for decades? ”

Lu Yuan kicked over angrily and scolded: "A guy who is blind and learns a few words is the same as going to the execution ground, he actually dares to point fingers here, are you eating too much grain noodles and opening a yellow cavity?" I'm not afraid of the doctors, they joke ......"

Wang Ma Gong, Big Dog Bear and the others all shrank their necks and stopped talking, but they muttered in their hearts-Didn't you say it yourself, the head of the regiment, that is, penicillin? Is this a lesson for us?

"The head of the regiment is right, it's not that simple!"

Dr. Wang said: "These plaques seem to be exactly the same, but under the microscope, they are composed of countless bacteria, among which there are many impurities, if they are used by the wounded in this way, it is estimated that not only can not save them, but will make their wounds worsen faster - if you want to make these plaques into medicines, you must purify the most useful substances, and then start pathological experiments from animals to determine that there are no other side effects, as well as clinical dosage and so on. Only then can we achieve the purpose of curing the disease and saving people......"

Hearing Dr. Wang's explanation, everyone suddenly realized.

These truths that later generations of three-year-old children will understand, for these reckless men who know a lot of big words, if they don't explain it to them clearly, they won't understand what is going on at all.

However, Dr. Wang's introduction was obviously a word in his words - he was not sure that there was actually penicillin in the plaques that Lu Yuan said, and the effect of penicillin in controlling inflammatory complications was as good as he said.

"Doctor, you believe me!"

Lu Yuan nodded confidently at Dr. Wang and the others: "Do what I say, and you will definitely be able to produce penicillin!" ”

Large-scale production of penicillin, even if you know how to produce it, you will definitely not be able to produce it now.

However, first extracting some penicillin to save Chen Ergouzi's seriously injured people, and making sure that penicillin is indeed useful, Lu Yuan is sure that this is feasible - of course, first of all, those wounded can last long enough.

What he is now preparing for Dr. Wang and others to use is to prepare penicillin by the soil method.

The first thing to do in the preparation of penicillin by the soil method is to collect a large amount of penicillium, that is, the orange peels with plaque that Lu Yuan asked the cow pimple to find, and there is a large amount of penicillium on them.

Next, the rice is ground into rice milk mixed with the juice boiled from taro as the culture medium, and then these penicillium plaques are implanted and cultured for seven days to allow these active penicillium to multiply.

After seven days, the culture is filtered to remove impurity precipitates.

In the culture medium that filters out the sediment, rapeseed oil is added to dissolve the fat-soluble substances, that is, the residual substances in the rice milk taro juice, and then the oil floats and the water juice sinks to achieve stratification.

Since penicillin is water-soluble and never mixes with oil, the underlying water will be saturated with penicillin.

The isolated solution full of penicillin is extracted, and the toner powder that has been boiled and sterilized at high temperature is stirred, and the adsorption of the toner will adsorb the penicillin in the solution to the toner.

Filter out the toner, then put the toner into a separator tube with a large opening at the top and a small opening at the bottom, and control the liquid flow rate with a filter cotton below.

Wash with distilled water and inject acidic water, and finally alkaline water.

The result is a near-pure penicillin, which extracts pus from the wounded wound.

Wound pus contains a large number of staphylococcus, and the mass reproduction of staphylococci is the root cause of wound inflammation.

The purified penicillin solution is cultured with these pus, and if the staphylococci in the pus only grows around the penicillin and does not invade the range of the penicillin purified solution, it is considered to be successfully purified.

Of course, the different concentrations of penicillin in these purified solutions will greatly affect the effectiveness of inflammation control, and more testing is required to ensure the effect, and a lot of time is required for experimentation.

Moreover, penicillin has side effects, especially for patients who are allergic to penicillin, and a small dose of skin allergy test is required to confirm that the constitution is not allergic before it can be used.

In short, the local method of preparing penicillin has been demonstrated to be feasible, but if it really wants to act on the wounded, I am afraid it is not possible to do it in a short time.

During the operation process, the people present were in a fog.

Big Dog Bear Bull Pimple Xu Yunluo Youcai These old hats are wide-eyed, what kind of culture medium, what kind of water-soluble, etc., dizzy, looking at Lu Yuan's eyes is like looking at a fairy, and at the same time his face is full of suspicion, as if asking, Leader, what you said is a set of sets, but is it okay?

However, Pan Haodi, Zou Leping, and the knowledgeable experts were a little skeptical when they heard this, and they couldn't help but look at the expressions of Dr. Wang and the others, trying to determine whether Lu Yuan's words were feasible.

And the three of Dr. Wang were already trembling with excitement, and the two assistants were holding pens and small notebooks to record, and exchanged two sentences with each other from time to time, for fear of missing a word!

Their expressions were full of shock, questions, and all kinds of indescribable expressions!