798, penicillin
What Bai Shujie wants to impress Payne is that he started cultivating it three years ago, began small-batch extraction a year ago, and only really entered the clinical trial of penicillin G when he arrived in Pyongyang.
It is not difficult to cultivate penicillin and concentrate penicillin, but the difficult thing is the preparation of nutrient bases, the control of ambient temperature, batch culture, extraction, purification, drying and preservation processes.
At that time, in Chengde, there was no industrial capacity to produce compressors, let alone chemical factories to produce foaming agents, so there was no way to produce air conditioners, and naturally there was no way to control the constant temperature state.
However, the seeds produced by coccus are mixed with O.5%-1.0% corn syrup by cryovials, and then implanted in triangular flasks to cultivate the original rice spores, and then moved into special culture flasks to produce rice spores (also known as production rice) in batches, and the parent rice and production rice are both 25 ° static culture.
There are no difficulties in the production process, but the difficulties faced by Bai Shujie are mainly in terms of equipment and instruments.
The first is the biological microscope, and the supporting color filter needs to be complete. After the occupation of Chengde, two microscopes were found in several hospitals, but neither was practical.
Later, when Bai Shujie's soldiers were trapped in Shentouling and his life was hanging by a thread, Zhao Jinxi was so angry that he ordered Yang Erya's special team to launch the first devastating blow against the little devils in the Pingjin area.
That war dealt with all the little devils in the Japanese Concession in Tianjin, regardless of men, women, and children. As for the little devils, the major hospitals were looted, and after all kinds of medicines and medical equipment were shipped back to Chengde, they were piled up.
Bai Shujie escaped death and was rescued back to Chengde, and found two microscopes made in Germany and one microscope made in Sweden among the items that Yang Erya snatched back.
After comparison, the microscope produced in Sweden was confiscated by Bai Shujie, and the two microscopes produced in Germany are now used in field hospitals.
With a high-quality standard microscope, it is possible to observe various changes such as the generation, growth, and enrichment of filamentous bacteria and cocci at any time, so as to have the conditions for recording the most complete set of numbers from scratch, so as to develop a standard process. finally triggered Bai Shujie's idea of producing penicillin.
The first type of equipment that Bai Shujie needed was a distillation facility capable of producing water for injection.
Penicillin produced by the "freeze-drying method" is white powdered granules. Penicillin cannot be dissolved in glucose, and when it is given intramuscularly or intravenously, it must first be melted in water.
There is no condition to save it in Chengde, so I can only make a little bit of it temporarily every time to ensure that the officers above the battalion commander of the Rehe Front can use a 5,000 unit of penicillin after being injured!
The second set of equipment that Bai Shujie needs is a high-power air-conditioning equipment that can control the constant temperature state, which has been bothering him.
In a big war not long ago, a good thing fell from the sky -- the world's first high-speed train "Asia" limited express train newly produced by the little devils!
Because there is no railway, Bai Shujie can only endure the pain of this luxury train and "give" it to Chengde. But he did one thing, which was to dismantle the large air-conditioning equipment on the train!
After coming to Pyongyang, why did Bai Shujie first order Zhang Yushu, the "mayor of Pyongyang", to quickly dig a cave with grain as wages, and ask for as deep as possible?
It turns out that in order to get the best quality and highest yield of coccoli, the pro-rice and the production of rice sporospores need to be stored in the zà i 5°C refrigerator.
Bai Shujie has neither a refrigerator nor a freezer. He is able to make a simple dehumidifier to ensure humidity in a separate space.
Now there is a high-power refrigeration system, but to ensure a constant temperature, it is better to be in a cave. At the same time, it is convenient for confidentiality and security guards. Bai Shujie knew that once penicillin came out, there would be no trouble in the future.
Because Bai Shujie drilled into the cave whenever he had time, Huang Qiaoyun, the head of the guard regiment, said last time that he was not doing his job: the good headquarters did not stay, but he wanted to run into the cave, and outsiders were not allowed to follow.
The third problem that Bai Shujie wants to mass-produce penicillin is the problem of packaging carriers! To put it bluntly, it is a small glass bottle that can be "fully sealed" and "easy to transport".
There is no glass factory in Chengde, there is no rubber factory. Even if a few replacement glass bottles were found, it was not possible to produce a non-toxic rubber stopper. I once thought about using wax pellet cork to seal it, but the effect was not good.
Robbing Jinzhou, Yingkou and Lushun, Bai Shujie got an alcohol factory, a soda ash factory, a nitric acid factory, a sulfuric acid factory, a rubber factory, a glass factory, a cotton yarn factory, and most importantly, a small steelmaking factory.
With these factory equipment, Bai Shujie does not need to mass-produce anything that supplies the market. The first thing he has to do is that the conditions for his "PH calibration" are finally ripe.
The culture of penicillin strains must be carried out under weak acid conditions, and how to be a standard weak acid environment, which is to calibrate the "pH". The easiest way is to make a PH test strip.
The strains are all readily available, and now there are basic conditions. So when we arrived in Pyongyang, it was "everything is ready, and the east wind is blowing again!" ā
Wan Haotian's "special medical team", with Bai Shujie chasing his ass every day, finally added more than 320 young students and began to operate at full capacity.
It was precisely because of the need to ensure a relatively stable environment that Bai Shujie sent a wing to Kawagishi Bunzaburo's 20th Division to "shamelessly provoke" and issued an unprecedented order that "it is not allowed to expand the scale of the battle without restrictions".
If it weren't for Bai Shujie killing little devils before, it would be like cutting winter melon. If he "wants to betray the country, no one dares to buy it", the commanders and fighters of the whole army are likely to suspect that Bai Shujie has become "corrupt and degenerate" and wants to be someone Jiang.
In the blink of an eye, it had been a month since the Flying Dragon Expeditionary Force entered Korea, and the first process of penicillin was finally completed, and the real sense of the word, that is, penicillin that could be used in large quantities for clinical trials - 240 sticks of 10,000 units, equivalent to 480 sticks of the original 5,000 units.
The book explains that in order to make its clinical trials convincing, the field hospital's "special medicine room" was divided into six areas, which housed more than 1,000 wounded from the last war, as well as "little devil captives" of different genders and ages.
Bai Shujie took Payne to this real place, which is the clinical real yĆ n area from 5 to 60 years old. The symptoms include tuberculosis patients who can only wait for death, patients with Bacillus anthracis who cause deep ulceration of the skin, and a group of "syphilis" patients.
These three categories are all "terminal diseases" that cannot be cured at all in this era.
Even the top person in charge of the special medical team, Wan Haotian was not sure whether he could cure these three kinds of patients. But with the deepening of the medical process, the patient's condition is getting better and better.
Payne looked at the scene in front of him and exclaimed: "This is the gospel of the whole world, and it is the first miracle of mankind!" ā
He is a businessman, with an agile sense of the market and, of course, an extraordinary ability to react.
When he saw so many patients who were "contagious" and had not been infected, he asked to see the clinical records.
Wan Haotian strictly abided by the confidentiality regulations, so naturally he couldn't agree, but intentionally or unintentionally set his sights on Bai Shujie.
Bai Shujie had another purpose, and at the same time, he knew in his heart that it was impossible to leak secrets just by looking at the medical records, so he secretly nodded in agreement.
Payne carefully went through almost all the medical records, and then examined them one by one, and only after two hours did he ask in a deep voice, "Your Excellency Commander, can I take a batch of pictures here?" This is key for future marketing! ā
Sure enough, it is worthy of the old fritters rolling in the international trade market!
Bai Shujie was secretly happy in his heart, but his face was indifferent: "Mr. Payne, I think you should understand that this place of mine belongs to a place of absolute secrecy. You are the first outsider in the world to enter this core area! ā
"However, because of the previous trading process, I think you are a real friend you can trust, so I will bring you over for a tour. That being the case, I don't have anything to keep secret from you. Except for me and this Dr. Wan, you can shoot casually! ā
Back at the headquarters, Payne was already a little impatient: "Mr. Commander, I apologize for what I said earlier!" The mysterious Orient not only has a long cultural heritage, but also the world's leading scientific and technological inventions and industrial innovations. I think that the benevolent Mr. Commander invented this special medicine and would not care about benefiting more people! ā
Bai Shujie nodded slightly: "Mr. Payne, you have been here for many years, you should know that the Chinese nation is a peace-loving nation, and their ancestral motto is to be kind to others. If it weren't for the damn Japanese dwarf, we wouldn't have risen up to fight. ā
Speaking of this, she beckoned to the outside world, Wang Xinlan came in with two information bags, and Bai Shujie then continued: "Let me introduce you, this Miss Wang is my special and plenipotentiary assistant in this project." I invited Mr. Payne to come here mainly for this purpose, because I need to register patents in the Americas and Europe at the same time. ā
"Mr. Payne, once the patent application is approved, you will be the general agent in the Americas from now on. If we cooperate for a long time, in order to reduce costs, when the conditions are right, we can invest in your hometown to set up a factory. Rest assured, I will not trade with anyone in the Americas but you. ā
When Payne heard this, his eyes lit up, because he had discovered the vast market "money scene" of this special drug.
Now is the time to pull the relationship, so Payne threw down Bai Shujie and began to get close to Wang Xinlan: "King Mister, I hope we will have a happy cooperation in the future!" ā
Wang Xinlan smiled elegantly: "Mr. Payne, as long as you can meet one condition, we can set off tomorrow, and we will probably be able to catch Christmas in your hometown!" ā
Payne rubbed his hands together and said, "Beautiful young lady, please command, what conditions do you need me to meet?" ā
"Mr. Payne, this special medicine was developed by our commander-in-chief to alleviate the pain of the soldiers' injuries. In addition to being able to reduce inflammation urgently, its biggest effect is actually to save the lives of soldiers on the battlefield. ā
Speaking of this, Wang Xinlan's face suddenly became cold and abnormal, and her words were almost word for word: "The Japanese militarists are invading my motherland, and they are our enemies. My condition is that as long as there is still a Japanese invader in my land, this medicine cannot be sold to Japan, not a single one! ā
After all, what happens next, let's see how it will be broken down next time.