Now that the experimental materials are abundant, the next step is to design a feasible experimental protocol.

The file of the Uchiha clan's writing wheel eye was sealed by Konoha, and the writing wheel eye is actually a secret, and even Sasuke's gradual promotion of the eye is all based on his own step-by-step exploration.

Today's Sasuke Sharingan is a double-eyed double-hooked jade sharingan, in order to allow Naruto to better help him develop the Sharingan, he told the truth about his eye-opening experience, and Naruto recorded it.

Sasuke's eyes, which went from ordinary pupils to single hook jade, were caused by the fact that his family was killed, but he was powerless in the face of his brother who killed his parents, and he was extremely emotional.

The advancement of single magatama to double magatama was during the mission of the country of waves, during the battle with Shiro, the situation was extremely urgent, and he was facing a life-and-death crisis, and this pressure forced him to advance to double magatama with one eye and see Bai's movements clearly.

The simultaneous advancement of the double magatama with both eyes was not long ago, when he fought with Orochimaru in the middle ninja examination room, he was also facing a great crisis of life and death.

That's the end of Sasuke's experience in writing the advanced wheel eye, how to open the three hooks in the future, and how to change the three hooks in the future, and how to advance to a kaleidoscope, Sasuke himself doesn't know.

The information provided by Sasuke is insufficient, but Naruto still has intelligence channels in the country of dragons, and the information provided by the country of dragons is somewhat richer than Sasuke's, but it is also limited.

The mechanism of the promotion of the double hook jade to the triple hook jade has not changed much, and it is also facing great pressure, encountering a life and death crisis, and there is a chance of promotion if the mood is extremely tense.

When Sangoyu was promoted to Kaleidoscope Writing Wheel Eye, the mechanism changed, and the condition required was to witness the death of a loved one in front of him.

That's the end of the information in the country of dragons, and there are no advanced conditions for kaleidoscope writing wheels.

After all, there are only nine people in the history of the ninja world who have Kaleidoscope Sharingan, and there are only two people who have Eternal Kaleidoscope Sharingan, one of whom is Sasuke, even if Luo Feng knows the plot, he can't spoil it before it happens.

Among the people who have a kaleidoscope writing wheel eye are Sasuke's father Uchiha Fugaku and brother Uchiha Itachi, which is well documented, and spoilers won't cause much suspicion.

The current Sasuke is still a double hook jade writing wheel, and Naruto's goal is to help him promote to the Kaleidoscope Writing Wheel, and the Eternal Kaleidoscope Writing Wheel Eye, which has not yet appeared, is not in Naruto's consideration at all.

After carefully analyzing the data, Naruto preliminarily deduced that the eye-opening and advancement of the Sharingan was a biological stress response and self-protection mechanism.

The Uchiha clan was promoted from single magatama to triple magatama when they were faced with great pressure that threatened their lives.

This is as if the gecko faced the predator, the body had an instinctive response to the danger, the tail muscle contracted urgently, the tail fell off automatically, and the detached tail nerve has not yet died, which can shake to attract the attention of the predator, so that the gecko can get a chance to escape.

The same stress defense mechanism, many animals also have, such as opossums in the face of crisis stimulation will play dead, this kind of play is not a kind of acting, but the body has a special reaction, the vitality is instantly cut off, the heart rate and breathing rate are greatly reduced, and a rancid smell will be secreted.

The special gene of the Uchiha family, that is, the blood inheritance limit, is likely to have recessive genes in the gene fragments, and when facing a life-and-death crisis, the eye cells are affected by the fear pheromones secreted by the amygdala of the brain and mutate, and the recessive genes begin to work and transform into dominant.

The amygdala is a brain tissue that produces emotions, recognizes emotions and regulates emotions, and controls learning and memory.

If a member of the Uchiha clan suffers from a disease like that of a madman who doesn't know he's crazy, he's afraid he won't be able to open his eyes for the rest of his life.

After proposing a hypothesis, the next step is to design an experiment based on the hypothesis to verify whether the hypothesis is valid.

At this point, Naruto made another difficulty, if it was the Minke Orochimaru who designed the experiment, the matter would be simple, just go to the war-torn country to capture some innocent people, and then brutally torture and intimidate the innocent people, kill them at the moment when he was most frightened and have the strongest desire to survive, cut open the brain, extract the brain tissue fluid, and then inject the brain tissue fluid into the culture jar containing the eyes of the Uchiha clan, and observe whether the eyes will change.

After starting to study life science, Naruto's temperance has been lost a lot, but the bottom line is still there, but he can't do such a cruel dark human experiment.

The biopheromone secreted by the amygdala is very complex, and there is neither a molecular formula nor an artificial synthesis, so how to make it becomes a problem.

In this way, there was a bottleneck in the research, and Naruto racked his brains and couldn't come up with a solution, so he had to apply for scientific and technological assistance from the Dragon Country.

Mr. Braderow was very supportive of Naruto's proposal to develop the Sharingan, and immediately sent his chief secretary, Io, a grim man with glasses.

Listening to Naruto's account of the problems he faced, Io was disdainful of his rejection of cruel human experiments, but due to the fact that the Dragon Country had always regarded itself as a human rights defender, he agreed to help him design an amygdala phobic hormone extraction program that did not violate human rights.

Io took out a biochemical brain, this biochemical brain is a prop in the world of "Fallout 2", if the protagonist has high technology points, he can upload his memories to the biochemical brain, and then perform brain replacement surgery, replacing the brain with a biochemical brain, which can increase two intelligence attributes.

This biochemical brain is a human brain that has been washed away from its memories, and a chip is installed on the brain, which greatly improves the speed of thinking.

Immediately afterwards, Io took out a large quantum supercomputer, which is from the universe of "The Matrix", which can virtualize a highly realistic matrix world through the back of the head, and it is difficult for people in the matrix world to distinguish the boundary between reality and virtuality.

Connecting the biochemical brain to the Matrix World supercomputer, Ai Ou took out a few memory storage chips that he got out of nowhere, and sealed the complete memory data of a normal person in these chips, and then uploaded the data in the chip to the biochemical brain chip, creating a bionic person with only a brain.

The consciousness of the android lives in the server of the matrix world, and the brain is equivalent to a personal computer with an online game client installed, although everything that happens in the matrix world is virtual, but the amygdala of the bionic brain can secrete fear pheromones under virtual stimulation.

This device is a small fear pheromone factory, which can continuously produce fear pheromones of various chemical compositions, and the specific mediation method is to simulate all kinds of terrorist events in the matrix world, so that the bionic brain is as frightened as if it has experienced it in real life.

In the matrix world, the bionic brain may experience terrifying scenes such as the killing of the whole family, being cornered by strong enemies, being hunted by monsters, etc., such stimuli force the amygdala to secrete special chemicals, and then collect these chemicals to purify, which becomes an eye-opening catalyst for writing wheels.

After getting the purified fear hormone, the materials for the Sharingan Eye-Opening Experiment were complete, and Naruto was finally able to officially conduct the Eye-Opening Experiment in vitro.

Taking a jar with a pair of eyes on it, Naruto used a pipette to pull out a small amount of a highly concentrated fear hormone solution and injected the solution into the jar.

According to the previous inference, the biochemical environment of the human body when a person with a writing wheel eye encounters a huge stimulus has been simulated in the writing wheel eye culture medium, and the ordinary eyeballs should be able to open their eyes, and they will be advanced to a single hook jade writing wheel eye.

However, after waiting for a long time, nothing happened!

What's going on?

Naruto tried to increase the dose of the fear hormone, but as a result, his eyeballs had a toxic reaction, became tarnished, and he went blind!

A pair of Uchiha blood-blooded eyeballs cultivated from embryonic stem cells were so easily discarded.

Naruto was disappointed, but also a little lucky, fortunately he didn't directly experiment on Sasuke, otherwise it would be Sasuke who would be blind!

There is nothing to be sorry for scrapping a pair of mass-produced eyes, with embryonic stem cells that can increase the value of the eye, as many pairs of eyes can be cultivated.

Casually throwing the eyes into the biochemical waste bucket, Naruto took out another can, and this time instead of dripping with the fear hormone, he began to think.

What's the problem?

The human environment is already highly simulated, why is it that the eyes still can't be promoted, but are blind?

Altitude Simulation ...... Is it that the realism is still not enough, and some necessary elements are missing?

Naruto thought about it, and suddenly a flash of inspiration occurred in his mind!

Chakra!

The Uchiha clan is a ninja family, everyone is a ninja, they have all practiced ninjutsu, and they definitely have chakra in their bodies.

Moreover, at the moment when the eyes of the writing wheel are opened, the Uchiha people are either cultivating or fighting, and the chakra content in the body is definitely not low!

The chakra in the jar, even if it is stimulated by fear hormones, lacks the flow of chakra in the cells, of course, it will not be advanced, and the cells will lose their activity.

Then, in the next experiment, it is necessary to increase the injection of chakra, place the eyeball in the culture medium environment of chakra flow, and then carry out fear hormone stimulation.

Naruto pressed his hand on the glass jar, and after learning Spiral Pill's training, he was already proficient in controlling the condensation and rotation of chakra particles in the palm of his hand, not to mention the technique of injecting chakra into objects.

The particle accelerator under the scientific ninja research and development center was built to study the changes in the nature of chakra, and Naruto also sent shadow clones to carry out chakra particle collision experiments half a year ago, and now he can transform chakras of any nature, the difference is that he is currently good at wind escape, and he can also make other system escape chakras at that time, but the conversion rate is very low.

Considering that the Uchiha clan is generally good at fire escape, Naruto first chose to inject fire escape chakra, and when the chakra filled the entire jar, he used a pipette gun to fill the culture medium with trace amounts of fear hormones.

As expected, a change has happened!

The eyes in the jar began to turn red and bloodshot, and sure enough, the fire chakra and the fear hormone worked together to cause the cells to mutate!

However, the pupils of the eyes did not appear Magatama, and the bloodshot became denser and denser, and then with a "boom", those eyes exploded!

Fortunately, the explosion was not strong, and the glass of the jar that burst open only cut Naruto's cheek.

Once again, the experiment failed.

Wiping the blood from his cheeks, Naruto was not discouraged at all, failure is also success in a sense, trial and error is the only path to success in scientific research.

There are only seven chakra nature changes in total, and even if the fire escape fails, it will definitely change if it tries six times at most.

After briefly cleaning up the test bench, Naruto took another jar of eyeballs and tried the other four-series retreat chakra.

When injected into the Thunder Chakra, the eyeballs glow blue, but they quickly burn black.

Ice Escape Chakra's eyeballs froze and shattered.

Wind escaped Chakra's eyeballs tore into pieces.

Tu Dun Chakra's eyeballs turned into stones.

At this point, all the conventional five-series retreat techniques failed.

Is it only the Yin Escape or Yang Escape Chakra that is the right choice?

What Naruto is not good at is that the nature of the Yin Chakra has changed, and the physical strength conversion rate is extremely low, approaching zero.

He was better at Yang Dun, so he started the test from Yang Dun, and after Yang Dun Chakra entered, his eyeballs began to proliferate, growing into a winged eyeball monster!

The eyeball monster burst through the glass jar, flapped its wings and flew up, hovering around the laboratory like a large, one-eyed bat.

This scared Naruto a lot, and let out a little girl's scream like a bug, but fortunately the scientific monster he created didn't have a mouth, but it couldn't bite, and it flew aimlessly for a while before running out of vitality, and fell to the ground with a thud and died.

Even if he used the elimination method, Naruto could be sure that the Yin Chakra would work.

However, he is not good at the change of the nature of the chakra, and the development of the Sharingan experiment has once again reached an impasse.

Naruto is not good at Yin Escape Chakra Change, but there are many ninjas in the village who are good at Yin Escape, like Yamanaka Ino and Nara Shikamaru, and their family ninjutsu is all secret techniques powered by Yin Escape Chakra.

But Naruto got the Wheel Eye development experiment, which touched a lot of ethical taboos, the people of the Dragon Country are okay, they are more tolerant of life science research, and they can treat it with a scientific attitude, if the natives of Konoha Village knew that Naruto was secretly conducting these experiments, they would definitely treat him as the second Orochimaru.

In desperation, Naruto turned to his scientific mentor Bredrow for help, and Io returned to the lab once again.

The technique that Io used to seal the soul was Yin, a spiritual power that created a body out of the invisible.

The total amount of Io's Yin Chakra has reached the shadow level, and he is well aware of the changes in the nature of Yin Chakra.

With Io's help, Io was responsible for feeding the culture fluid into the Yin Chakra, and Naruto used a pipette gun to drip fear hormones, and the two fused together, and the nature of the Yin Chakra also changed strangely, turning into a special kind of Chakra.

Under the effect of this chakra, the pupils of the eyeballs soaked in the culture liquid suddenly dilate, and the original position of the pupils was replaced by red ringed stripes, and a magatama pattern appeared on the stripes.

The single hook jade writing wheel eye is a success!

Naruto's experimental design can't be said to be absolutely rigorous and perfect, but it can be regarded as crooked, and it just meets the sufficient and necessary conditions for writing wheels to open their eyes.

According to Senjujuma, when the Uchiha people understand the true meaning of "love" and then lose it, the emotion of "love" turns into a more powerful hatred.

In other words, when the Uchiha clan understands the pain of losing "love", a special chakra will invade their brains, causing nerves to grow regularly, causing their eyes to change abnormally.

This particular chakra is a variant of the yin chakra, which is produced by biochemical changes in the mood hormones secreted by the yin chakra and the amygdala.

Human emotions come from the amygdala of the brain, and people who have removed the amygdala do not have any emotions, and of course they do not feel love and hate, and the principle of the amygdala that makes people feel emotions is to secrete special biopheromones, that is, the "fear hormone" prepared by Naruto with the biochemical brain.

The combination of Yin Chakra and fear hormones stimulates the eye nerves of the Uchiha clan, causing physiological changes in the "eye opening".

Naruto breathed a sigh of relief, and the experiment finally made a breakthrough, successfully opening an ordinary eyeball carrying the Uchiha gene.

But this is only the first step of the ultimate goal, and the next step is to continue to study how to further advance the single magatama writing wheel eye, and finally upgrade to the kaleidoscope writing wheel eye.

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