When Yang Rui chooses the right degree, the right DNA length, and the right tracer, it is not difficult to make results.

In fact, the work of the vast majority of graduate students is to do known experiments, and to use known experimental methods to make results, which is the requirement of the laboratory for scientific research dogs.

The first year of the master's degree is usually dominated by theoretical study and laboratory exercises, and at this time, they may not be able to achieve results when they do a middle school experiment.

But in the second year, when the graduate students began to do experiments frequently, the speed and success rate of their experiments were greatly improved.

For experiments like the first-generation PCR, if a second-year graduate student is not proficient in it, he is not qualified to be called a scientific research dog, at best, a wild dog, wandering between the laboratory and the laboratory corridor.

Of course, Yang Rui himself has not been exposed to the first generation of PCR, and when domestic students are exposed to PCR, at least it is also the second generation of PCR, which is a very simple and common instrument, and the size and usage are similar to that of a microwave oven.

Compared with the second-generation PCR, the difficulty of the first-generation PCR is equivalent to that of an oven, and Yang Rui is familiar with it after doing so many experiments, and this time, he is serious about pretreating the raw materials, adding tracers to the raw materials, and seriously adjusting the temperature and time.

Then, Yang Rui seriously wrote "PCR128" in the experimental book.

However, everyone in the laboratory was serious, and Yang Rui, who was serious, attracted Wei Zhenxue's ridicule.

"Yang Rui, you are just in the experiment book, using the official book to make records, it's useless, you have to do experiments, right? Wei Zhenxue came to Yang Rui with a stroll. His workload is not large now, doing experiments is purely a personal hobby, and he has published several papers, and he is very relaxed.

Yang Rui was also in a good mood, and said with a smile: "How do you know that oligonucleotides don't listen to me." ”

Wei Zhenxue was inexplicably surprised, and suddenly asked in a low voice: "Can you make oligonucleotides listen to you?"

After speaking, he also looked behind him alertly, and said, "What is your qigong called?

Yang Rui was dumbfounded: "How can you think of qigong." ”

"Why can't you think of qigong? Look at you, you can't do it every day, how can you do it so seriously today, you must be doing it. Otherwise......" Wei Zhenxue dragged out a long voice, the corners of his eyes slanted, and said, "What kind of master did you ask for, right?"

Yang Rui's expression was as if he was being stared at by a dog, and he looked at Wei Zhenxue with anti-rabies eyes.

Wei Zhenxue thought that he had seen through Yang Rui and said with a smile: "That's right, I guessed it through, you wait, I guess who did it for you, Master Hu? He recently gave a lecture at the Temple of Heaven, but he was very busy...... That's Master Zhu, isn't that Master Zhu from Dongcheng?"

"Old Wei!" Yang Rui had to interrupt him, and looked at Wei Zhenxue with the eyes of a boss: "You have been quite idle recently, you have learned so many things." ”

"Live and learn. Wei Zhenxue smiled proudly.

"Learn qigong and use qigong to do scientific research?"

"Oops, I'm also in the experimental stage, hahaha......"

"Well, that's fine, in the future, you don't need to use the scientific research instruments in the laboratory, such as microscopes and the like, qigong testing, save a little instrument resources, so that everyone can be more relaxed, and you should give full play to your residual heat." ”

When Wei Zhenxue heard that he was not allowed to use the instrument, he was anxious and said, "What kind of exertion of residual heat, I'm not old yet." I'm just too young, that's why I haven't learned qigong well, I haven't learned qigong yet, Yang Rui, I still have to use instruments, I can't use qigong. ”

"Qigong can't work?"

"I can't use qigong now. ”

"Qigong can't work?"

"Hey, you're right, qigong doesn't work!" Wei Zhenxue adheres to the Chinese style of faith, and absolutely only chooses the advantageous ones.

Yang Rui lost Wei Zhenxue's two sentences, and suddenly felt that the blood circulation was dissipated, and the whole person was comfortable.

There really has to be a scolded person in the laboratory to be harmonious.

Driving Wei Zhenxue to the side, Yang Rui began to make the "PCR-128" of the simplified system.

Compared to experiments numbered 50 years ago, the reaction volume of PCR-128 was also reduced, and the reaction concentration was further increased.

Yang Rui carefully and carefully recorded all the experimental preparations and experimental materials, and then looked at the water bath in a daze.

At this point, Yang Rui's experiment is slightly different from the original PCR.

Because the original PCR does not have heat-resistant polymerase, it takes tens of seconds to come in and go out from the water bath......

With heat-resistant polymerase, this step can be omitted, and Yang Rui can be a little easier.

He counted the time, put things up, and started running electrophoresis.

The purpose of running electrophoresis is to see the radioactive tracer in the gel.

The gel is jelly-like, and the DNA that has gathered up as a result of electrophoresis forms bands on the gel.

If you get the band, it proves that there is a product in it, and if you don't get the band, the gel is still clean like fresh jelly, that is the absence of the product, which means that the experiment has failed.

Wei Zhenxue stood silently behind Yang Rui, his knees slightly bent, his hands stretched out, and his face was as red as constipation.

Yang Rui's attention was focused on the gel, and he didn't know that someone was watching behind him.

Yang Rui quietly waited for the electrophoresis to be completed, slowly pulled out the gel, and then placed it under the light of the experimental table to carefully observe the gel......

"Oh my God...... I succeeded, I made a strip!" Wei Zhenxue suddenly shouted like a pig, his palms facing him, an expression of disbelief.

Yang Rui was so frightened that he almost jumped up, and when he turned around and saw that it was Wei Zhenxue, he couldn't help but scold angrily: "What the hell is your name!"

"I just helped you do the work, and as a result, you made the strips, look, you see, the color of the strips is a little lighter, but if you make the strips, I send the work, I send the work!" Wei Zhenxue pointed to himself vigorously.

"Did you really make a strip?" Huang Mao pushed Wei Zhenxue away, looked down at the gel, and said, "In this way, your more than 100 experiments can be regarded as having survived." ”

Not only Huang Mao, everyone nodded with palpitations.

It is a cruel act to repeat the experiment more than a hundred times, and the researchers with weak psychological tolerance will collapse, and everyone is relieved to see that Yang Rui has finally made something.

Yang Rui also breathed a sigh of relief.

Although the correct experimental method was used, there were still cases where the results could not be made by looking at the experiment manual.

"I'll see, it's really made, and it's copied the same DNA, is that what it means?" Li Wenqiang leaned closer and looked at the gel seriously.

"I just did my job. Wei Zhenxue also got closer.

Li Wenqiang gently pushed him away and said, "Don't make trouble." ”

"I've been practicing for half a year. Wei Zhenxue came to Zhang Xuetong who came over to join in the fun.

"Good. Zhang Xuetong patted Wei Zhenxue, turned a corner, and went to see the gel made by Yang Rui.

"There is indeed a strip, you can see it, it is indeed made. Li Wenqiang nodded as he spoke, his expression not much changed from usual. After all, in his opinion, it should be a good paper, but it doesn't stop there.

Yang Rui re-examined the experiment, and after confirming it, he called Wang Lei over without hesitation and said, "Call Li Zhangzhen and ask him to find a patent attorney to come over as soon as possible! ”

"What lawyer?" Comrade Wang Lei, the new professional experimental dog, is still unclear about these words.

"Patent lawyer, if you don't understand, just call me first, and then call me. Yang Rui said and said: "I'll do another repeat test, Zhou Yuwen, go take out the camera in the cabinet and shoot me from beginning to end, don't miss a little detail, use the video recorder, you must pay attention when the tape is about to end, you can notify me in advance." ”

PCR doesn't have to worry about leaks, this technology is invaluable because it is easy to use and widely spread.

Compared with confidentiality, Yang Rui cares more about patents and honors.

In between, Yang Rui had to first determine the honor, and video was the most commonly used means of experimental recording in later generations, which was very convenient, simple and undoubtedly determined the attribution of the results.

There were no digital video cameras in '84, but Yang Rui didn't care about wasting videotapes.

Huang Mao knew that Yang Rui was often targeted, so he thought about it curiously for a while, but he didn't come up with a reason.

The newcomer Li Wentong and others feel that Yang Rui is making a big fuss, but the advantage of a private laboratory is that the boss is full of authority, and Yang Rui uses his own money to do things, and others have nothing to say.

……

m.MM provides reborn god-level scholars without pop-ups to read high-quality full-text chapters online,High-speed first release of the latest chapters,Higher text quality,If you think MM is good, please help us promote and recommend this site,Thank you for your support!Your every sharing and publicity is the driving force for our high-speed debut!